Keratinocyte differentiation promotes ER stress-dependent lysosome biogenesis.

ABSTRACT: Keratinocytes maintain epidermal integrity through cellular differentiation. This process enhances intraorganelle digestion in keratinocytes to sustain nutritional and calcium-ionic stresses observed in upper skin layers. However, the molecular mechanisms governing keratinocyte differentiation and concomitant increase in lysosomal function is poorly understood. Here, by using primary neonatal human epidermal keratinocytes, we identified the molecular link between signaling pathways and cellular differentiation/lysosome biogenesis. Incubation of keratinocytes with CaCl₂ induces differentiation with increased cell size and early differentiation markers. Further, differentiated keratinocytes display enhanced lysosome biogenesis generated through ATF6-dependent ER stress signaling,

SUBMITTER: Mahanty S 

PROVIDER: S-EPMC6425001 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature