Inhibition of extracellular proteases improves the production of a xylanase in Parageobacillus thermoglucosidasius.

ABSTRACT:

Background

Parageobacillus thermoglucosidasius is a thermophilic and ethanol-producing bacterium capable of utilising both hexose and pentose sugars for fermentation. The organism has been proposed to be a suitable organism for the production of bioethanol from lignocellulosic feedstocks. These feedstocks may be difficult to degrade, and a potential strategy to optimise this process is to engineer strains that secrete hydrolases that liberate increased amounts of sugars from those feedstocks. However, very little is known about protein transport in P. thermoglucosidasius and the limitations of that process, and as a first step we investigated whether there were bottlenecks in the secretion of a model protein.

Results

A secretory enzyme, xylanase (XynA1), was produced with an

SUBMITTER: Holland ATN 

PROVIDER: S-EPMC6425571 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature