Structure of a VHH isolated from a naive phage display library.

ABSTRACT: OBJECTIVE:To determine the X-ray structure and biophysical properties of a Camelid VHH isolated from a naïve phage display library. RESULTS:Single domain antibodies (VHH) derived from the unique immune system of the Camelidae family have gained traction as useful tools for biotechnology as well as a source of potentially novel therapeutics. Here we report the structure and biophysical characterization of a VHH originally isolated from a naïve camelid phage display library. VHH R419 has a melting temperate of 66 °C and was found to be a monomer in solution. The protein crystallized in space group P6522 and the structure was solved by molecular replacement to a resolution of 1.5 Å. The structure revealed a flat paratope with CDR loops that could be classified into existing canonical loop str

SUBMITTER: White B 

PROVIDER: S-EPMC6425610 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature