Optogenetic stimulation of G_s-signaling in the heart with high spatio-temporal precision.

ABSTRACT: The standard technique for investigating adrenergic effects on heart function is perfusion with pharmaceutical agonists, which does not provide high temporal or spatial precision. Herein we demonstrate that the light sensitive G_s-protein coupled receptor JellyOp enables optogenetic stimulation of G_s-signaling in cardiomyocytes and the whole heart. Illumination of transgenic embryonic stem cell-derived cardiomyocytes or of the right atrium of mice expressing JellyOp elevates cAMP levels and instantaneously accelerates spontaneous beating rates similar to pharmacological β-adrenergic stimulation. Light application to the dorsal left atrium instead leads to supraventricular extrabeats, indicating adverse effects of localized G_s-signaling. In isolated ventricul

SUBMITTER: Makowka P 

PROVIDER: S-EPMC6426906 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature