Diminished apoptosis in hypoxic porcine retina explant cultures through hypothermia.

ABSTRACT: Simulation of hypoxic processes in vitro can be achieved through cobalt chloride (CoCl₂), which induces strong neurodegeneration. Hypoxia plays an important role in the progression of several retinal diseases. Thus, we investigated whether hypoxia can be reduced by hypothermia. Porcine retinal explants were cultivated for four and eight days and hypoxia was mimicked by adding 300 µM CoCl₂ from day one to day three. Hypothermia treatment (30 °C) was applied simultaneously. Retinal ganglion, bipolar and amacrine cells, as well as microglia were evaluated via immunohistological and western blot analysis. Furthermore, quantitative real-time PCR was performed to analyze cellular stress and apoptosis. In addition, the expression of specific marker for the previously describ

SUBMITTER: Maliha AM 

PROVIDER: S-EPMC6427006 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature