Autaptic cultures of human induced neurons as a versatile platform for studying synaptic function and neuronal morphology.

ABSTRACT: Recently developed technology to differentiate induced pluripotent stem cells (iPSCs) into human induced neurons (iNs) provides an exciting opportunity to study the function of human neurons. However, functional characterisations of iNs have been hampered by the reliance on mass culturing protocols which do not allow assessment of synaptic release characteristics and neuronal morphology at the individual cell level with quantitative precision. Here, we have developed for the first time a protocol to generate autaptic cultures of iPSC-derived iNs. We show that our method efficiently generates mature, autaptic iNs with robust spontaneous and action potential-driven synaptic transmission. The synaptic responses are sensitive to modulation by metabotropic receptor agonists as well as potentiat

SUBMITTER: Fenske P 

PROVIDER: S-EPMC6427022 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature