Quantitative Proteomics Identification of Seminal Fluid Proteins in Male <i>Drosophila melanogaster</i>.

ABSTRACT: Seminal fluid contains some of the fastest evolving proteins currently known. These seminal fluid proteins (Sfps) play crucial roles in reproduction, such as supporting sperm function, and particularly in insects, modifying female physiology and behavior. Identification of Sfps in small animals is challenging, and often relies on samples taken from the female reproductive tract after mating. A key pitfall of this method is that it might miss Sfps that are of low abundance because of dilution in the female-derived sample or rapid processing in females. Here we present a new and complementary method, which provides added sensitivity to Sfp identification. We applied label-free quantitative proteomics to Drosophila melanogaster, male reproductive tissue - where Sfps are unprocessed, an

SUBMITTER: Sepil I 

PROVIDER: S-EPMC6427238 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature