LmrR: A Privileged Scaffold for Artificial Metalloenzymes.

ABSTRACT: The biotechnological revolution has made it possible to create enzymes for many reactions by directed evolution. However, because of the immense number of possibilities, the availability of enzymes that possess a basal level of the desired catalytic activity is a prerequisite for success. For new-to-nature reactions, artificial metalloenzymes (ARMs), which are rationally designed hybrids of proteins and catalytically active transition-metal complexes, can be such a starting point. This Account details our efforts toward the creation of ARMs for the catalysis of new-to-nature reactions. Key to our approach is the notion that the binding of substrates, that is, effective molarity, is a key component to achieving large accelerations in catalysis. For this reason, our designs are based on the

SUBMITTER: Roelfes G 

PROVIDER: S-EPMC6427492 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature