Evolutionary Changes after Translational Challenges Imposed by Horizontal Gene Transfer.

ABSTRACT: Genes acquired by horizontal gene transfer (HGT) may provide the recipient organism with potentially new functions, but proper expression level and integration of the transferred genes in the novel environment are not granted. Notably, transferred genes can differ from the receiving genome in codon usage preferences, leading to impaired translation and reduced functionality. Here, we characterize the genomic and proteomic changes undergone during experimental evolution of Escherichia coli after HGT of three synonymous versions, presenting very different codon usage preference, of an antibiotic resistance gene. The experimental evolution was conducted with and without the corresponding antibiotic and the mutational patterns and proteomic profiles after 1,000 generations largely depend on th

SUBMITTER: Bedhomme S 

PROVIDER: S-EPMC6427688 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature