MicroRNA-127 inhibits cell proliferation via targeting Kif3b in pancreatic ? cells.

ABSTRACT: MicroRNAs (miRNAs) have been implicated in ? cells dysfunction. Previous studies indicated that miR-127 was specifically abundant in ? cells and one of its target genes, Kif3b, promoted cell proliferation. However, the impact of the miR-127-Kif3b axis on ? cells remains unknown. In this study, we revealed that miR-127 level was declined both in islets from the mice with a high-fat diet and in MIN6 cells with elevated glucose treatment. The elevated level of miR-127 attenuated ? cell proliferation by repressing Kif3b expression without affecting apoptosis and cell cycle, and it dampened insulin secretion. Moreover, ? cell-derived miR-127 could also affect the islet endothelial cell-line, MS1, in vitro via the transfer of extracellular vesicles (EVs). Treating MS1 cells with the EVs secreted by MIN6 cells exhibited a higher ability in cell migration and tube formation. However, this effect was abolished by the miR-127 inhibitor co-cultured with EVs-treated MS1 cells. Thus, we define that miR-127 is a crucial regulator of insulin secretion and cell proliferation in pancreatic ? cells as well as a potential functional regulation factor in islet endothelial cells.

SUBMITTER: Shen Z 

PROVIDER: S-EPMC6428088 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature