Rewired cellular signaling coordinates sugar and hypoxic responses for anaerobic xylose fermentation in yeast.

ABSTRACT: Microbes can be metabolically engineered to produce biofuels and biochemicals, but rerouting metabolic flux toward products is a major hurdle without a systems-level understanding of how cellular flux is controlled. To understand flux rerouting, we investigated a panel of Saccharomyces cerevisiae strains with progressive improvements in anaerobic fermentation of xylose, a sugar abundant in sustainable plant biomass used for biofuel production. We combined comparative transcriptomics, proteomics, and phosphoproteomics with network analysis to understand the physiology of improved anaerobic xylose fermentation. Our results show that upstream regulatory changes produce a suite of physiological effects that collectively impact the phenotype. Evolved strains show an unusual co-activation of Pro

SUBMITTER: Myers KS 

PROVIDER: S-EPMC6428351 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature