The Rapid Methylation of T-DNAs Upon Agrobacterium Inoculation in Plant Leaves.

ABSTRACT: Agrobacterium tumefaciens has been foundational in the development of transgenic plants for both agricultural biotechnology and plant molecular research. However, the transformation efficiency and level of transgene expression obtained for any given construct can be highly variable. These inefficiencies often require screening of many lines to find one with consistent and heritable transgene expression. Transcriptional gene silencing is known to affect transgene expression, and is associated with DNA methylation, especially of cytosines in symmetric CG and CHG contexts. While the specificity, heritability and silencing-associated effects of DNA methylation of transgene sequences have been analyzed in many stably transformed plants, the methylation status of transgene sequences in the T-DNA

SUBMITTER: Philips JG 

PROVIDER: S-EPMC6428780 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature