Measuring Mobility in Chromatin by Intensity-Sorted FCS.

ABSTRACT: The architectural organization of chromatin can play an important role in genome regulation by affecting the mobility of molecules within its surroundings via binding interactions and molecular crowding. The diffusion of molecules at specific locations in the nucleus can be studied by fluorescence correlation spectroscopy (FCS), a well-established technique based on the analysis of fluorescence intensity fluctuations detected in a confocal observation volume. However, detecting subtle variations of mobility between different chromatin regions remains challenging with currently available FCS methods. Here, we introduce a method that samples multiple positions by slowly scanning the FCS observation volume across the nucleus. Analyzing the data in short time segments, we preserve the high tem

SUBMITTER: Di Bona M 

PROVIDER: S-EPMC6428914 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature