Selective high-resolution DNP-enhanced NMR of biomolecular binding sites.

ABSTRACT: Locating binding sites in biomolecular assemblies and solving their structures are of the utmost importance to unravel functional aspects of the system and provide experimental data that can be used for structure-based drug design. This often still remains a challenge, both in terms of selectivity and sensitivity for X-ray crystallography, cryo-electron microscopy and NMR. In this work, we introduce a novel method called Selective Dynamic Nuclear Polarization (Sel-DNP) that allows selective highlighting and identification of residues present in the binding site. This powerful site-directed approach relies on the use of localized paramagnetic relaxation enhancement induced by a ligand-functionalized paramagnetic construct combined with difference spectroscopy to recover high-resolution and

SUBMITTER: Marin-Montesinos I 

PROVIDER: S-EPMC6429603 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature