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Metabolic engineering of Escherichia coli for the production of butyric acid at high titer and productivity.


ABSTRACT:

Background

Several anaerobic bacteria produce butyric acid, a commodity chemical with use in chemical, pharmaceutical, food and feed industries, using complex media with acetate as a co-product. Butyrate titer of various recombinant Escherichia coli did not exceed 10 g l-1 in batch fermentations in any of the media tested.

Results

A recombinant E. coli (strain LW393) that produced butyrate as the major fermentation product was constructed with genes from E. coli, Clostridium acetobutylicum and Treponema denticola. Strain LW393 produced 323?±?6 mM (28.4?±?0.4 g l-1) butyric acid in batch fermentations in mineral salt medium with glucose as C source at a yield of 0.37?±?0.01 g (g glucose consumed)-1. Butyrate accounted for 90% of the total products produced by the culture. Supplementing this medium with yeast extract further increased butyric acid titer to 375?±?4 mM. Average volumetric productivity of butyrate with xylose as C source was 0.89?±?0.07 g l-1 h-1.

Conclusions

The butyrate titer reported in this study is about 2.5-3-times higher than the values reported for other recombinant E. coli and this is achieved in mineral salt medium with an expectation of lower purification and production cost of butyrate.

SUBMITTER: Wang L 

PROVIDER: S-EPMC6429758 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

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Metabolic engineering of <i>Escherichia coli</i> for the production of butyric acid at high titer and productivity.

Wang Liang L   Chauliac Diane D   Moritz Brelan E BE   Zhang Guimin G   Ingram Lonnie O LO   Shanmugam K T KT  

Biotechnology for biofuels 20190322


<h4>Background</h4>Several anaerobic bacteria produce butyric acid, a commodity chemical with use in chemical, pharmaceutical, food and feed industries, using complex media with acetate as a co-product. Butyrate titer of various recombinant <i>Escherichia coli</i> did not exceed 10 g l<sup>-1</sup> in batch fermentations in any of the media tested.<h4>Results</h4>A recombinant <i>E. coli</i> (strain LW393) that produced butyrate as the major fermentation product was constructed with genes from <  ...[more]

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