Metabolic engineering of Escherichia coli for the production of butyric acid at high titer and productivity.

ABSTRACT:

Background

Several anaerobic bacteria produce butyric acid, a commodity chemical with use in chemical, pharmaceutical, food and feed industries, using complex media with acetate as a co-product. Butyrate titer of various recombinant Escherichia coli did not exceed 10 g l^-1 in batch fermentations in any of the media tested.

Results

A recombinant E. coli (strain LW393) that produced butyrate as the major fermentation product was constructed with genes from E. coli, Clostridium acetobutylicum and Treponema denticola. Strain LW393 produced 323 ± 6 mM (28.4 ± 0.4 g l^-1) butyric acid in batch fermentations in mineral salt medium with glucose as C source at a yield of 0.37 ± 0.01 g (g glucose consumed)^-1. Butyrate

SUBMITTER: Wang L 

PROVIDER: S-EPMC6429758 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature