Genome-wide CRISPR screen reveals PSMA6 to be an essential gene in pancreatic cancer cells.

ABSTRACT:

Background

Despite its relatively low incidence, pancreatic ductal adenocarcinoma (PDAC) is a leading cause of cancer deaths because of the aggressive growth/metastasis of the tumor, the lack of early symptoms, and the poor treatment options. Basic research to identify potential therapeutic targets for PDAC is greatly needed.

Methods

We used a negative-selection genome-wide CRISPR screen to identify essential genes in the PANC-1 human pancreatic carcinoma cell line. We validated the top hits with follow-up siRNA screens, using the HPNE, HPAF-II, AsPC-1, and Mia PaCa-2 cell lines.

Results

The PSMA6 gene was an identified candidate hit after the CRISPR screen, siRNA validation screen, and siRNA deconvolution screen. Spheroid formation assays and flow cytometry analysis

SUBMITTER: Bakke J 

PROVIDER: S-EPMC6429770 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature