Isothermal microcalorimetry for thermal viable count of microorganisms in pure cultures and stabilized formulations.

ABSTRACT:

Background

Quantification of viable microorganisms is an important step in microbiological research as well as in microbial product formulation to develop biological control products or probiotics. Often, the efficiency of the resulting product is dependent on the microbial cell density and their viability, which may decrease over time. Commonly, the number of viable cells is determined by serial dilution and plating techniques or flow cytometry. In 2017, we developed a mathematical model for isothermal microcalorimetry (IMC) data analysis and showed that the new method allows for a more rapid quantification of viable fresh and freeze-dried anaerobic Lactobacillus reuteri cells than traditional viable count methods.

Results

This study developed the new method further by appl

SUBMITTER: Nykyri J 

PROVIDER: S-EPMC6429831 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature