Functional histamine H₃ and adenosine A_2A receptor heteromers in recombinant cells and rat striatum.

ABSTRACT: In the striatum, histamine H₃ receptors (H₃Rs) are co-expressed with adenosine A_2A receptors (A_2ARs) in the cortico-striatal glutamatergic afferents and the GABAergic medium-sized spiny neurons that originate the indirect pathway of the basal ganglia. This location allows H₃Rs and A_2ARs to regulate the striatal GABAergic and glutamatergic transmission. However, whether these receptors can physically interact has not yet been assessed. To test this hypothesis, a heteromer-selective in vitro assay was used to detect functional complementation between a chimeric A_2AR₃₀₂-Gα_qi4 and wild-type H₃Rs in transfected HEK-293T cells. H₃R activation with the agonist RAMH resulted in

SUBMITTER: Marquez-Gomez R 

PROVIDER: S-EPMC6429923 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature