Determination of phosphatidylethanol 16:0/18:1 in whole blood by 96-well supported liquid extraction and UHPLC-MS/MS.

ABSTRACT:

Background

Phosphatidylethanols (PEths) are specific, direct alcohol biomarkers that can be determined in human blood to distinguish between heavy and social drinking. PEth 16:0/18:1 is among the most predominant PEth homologues in human blood. The aim of the study was to develop a high throughput and sensitive UHPLC-MS/MS method for the determination of PEth 16:0/18:1 in whole blood.

Methods

Whole blood samples were prepared by 96-well supported liquid extraction (SLE). Extracted samples were analyzed for PEth 16:0/18:1 by reversed phase UHPLC-MS/MS.

Results

The developed UHPLC-MS/MS method was fully validated in whole blood with PEth 16:0/18:1-D₅ as internal standard. Intermediate precision and intermediate accuracy were within ≤± 12% and ≤± 17%, respecti

SUBMITTER: Berg T 

PROVIDER: S-EPMC6430335 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature