A SimpleProbe^® real-time PCR assay for differentiating the canine parvovirus type 2 genotype.

ABSTRACT:

Background

Canine parvovirus type 2 (CPV-2) causes an important canine viral disease worldwide. CPV-2 belongs to the Protoparvovirus genus in the family Parvoviridae. An amino acid change at position 426 of the VP2 protein differentiate types of CPV-2, designated as CPV-2a (Asn), CPV-2b (Asp), and CPV-2c (Glu). In this study, we compared CPV-2 genotyping results obtained by SimpleProbe^® real-time PCR and DNA sequencing analysis to identify the accuracy and sensitivity of these methods.

Methods

One hundred rectal swabs were collected from CPV-2 naturally infected dogs from 2015 to 2017 at the Animal Disease Diagnostic Center, National Pingtung University of Science and Technology. CPV-2 genotyping was performed by SimpleProbe^® real-time PCR and DNA seque

SUBMITTER: Hoang M 

PROVIDER: S-EPMC6430354 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature