LncRNA NEAT1 promotes the tumorigenesis of colorectal cancer by sponging miR-193a-3p.

ABSTRACT:

Objectives

LncRNA nuclear-enriched abundant transcript 1 (NEAT1) participates in the development and progression of multiple malignancies. However, the molecular mechanism by which NEAT1 contributes to colorectal cancer (CRC) remains unclear.

Methods

The association between lncRNA NEAT1 expression and clinicopathological characteristics and prognosis in patients with CRC was analysed by TCGA RNA-sequencing data. MTT, colony formation, flow cytometry, transwell assays and a xenograft tumour model were used to assess the functions of NEAT1. Bioinformatics and spearman correlation analysis were used to identify the NEAT1-specific binding with miRNAs, and luciferase gene report and RIP assays were performed to confirm the interaction between miR-193a-3p (miR-193a) and NEAT1 in C

SUBMITTER: Yu HM 

PROVIDER: S-EPMC6430453 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature