IL-33 guides osteogenesis and increases proliferation and pluripotency marker expression in dental stem cells.

ABSTRACT:

Objectives

Soluble IL-33 (interleukin (IL)-1-like cytokine) acts as endogenous alarm signal (alarmin). Since alarmins, besides activating immune system, act to restore tissue homeostasis, we investigated whether IL-33 exerts beneficial effects on oral stem cell pull.

Materials and methods

Clonogenicity, proliferation, differentiation and senescence of stem cells derived from human periodontal ligament (PDLSCs) and dental pulp (DPSCs) were determined after in vitro exposure to IL-33. Cellular changes were detected by flow cytometry, Western blot, immunocytochemistry and semiquantitative RT-PCR.

Results

IL-33 stimulated proliferation, clonogenicity and expression of pluripotency markers, OCT-4, SOX-2 and NANOG, but it inhibited ALP activity and mineralization in both PD

SUBMITTER: Kukolj T 

PROVIDER: S-EPMC6430470 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature