Toward a Sensible Single-antigen Bead Cutoff Based on Kidney Graft Survival.

ABSTRACT:

Background

There is no consensus in the literature on the interpretation of single-antigen bead positive for a specific HLA antibody.

Methods

To inform the debate, we studied the relationship between various single-antigen bead positivity algorithms and the impact of resulting donor-specific HLA antibody (DSA) positivity on long-term kidney graft survival in 3237 deceased-donor transplants.

Results

First, we showed that the interassay variability can be greatly reduced when working with signal-to-background ratios instead of absolute median fluorescence intensities (MFIs). Next, we determined pretransplant DSA using various MFI cutoffs, signal-to-background ratios, and combinations thereof. The impact of the various cutoffs was studied by comparing the graft survival

SUBMITTER: Wisse BW 

PROVIDER: S-EPMC6430591 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature