Comparison of gene expression in cynomolgus monkeys with preclinical type II diabetes induced by different high energy diets.

ABSTRACT:

Background

Cynomolgus disease models that are similar to the preclinical stage of human type 2 diabetes mellitus (T2DM) were established by feeding middle-aged cynomolgus monkeys different high energy diets to study the differential expression of diabetes-related genes.

Methods

A total of 36 male monkeys were randomly divided into four groups and fed human diets with high sugar, high fat, double high sugar and fat, and a normal diet. The preclinical diabetes phase was determined by monitoring the metabolic characteristic indices and the results of oral glucose tolerance tests (OGTT). The mRNA expression of 45 diabetes-related genes in peripheral blood leukocytes was analyzed using real-time PCR.

Results

A total of 22, 25, and 21 genes were significantly up-regulated (

SUBMITTER: Jin LS 

PROVIDER: S-EPMC6431119 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature