Domain topology, stability, and translation speed determine mechanical force generation on the ribosome.

ABSTRACT: The concomitant folding of a nascent protein domain with its synthesis can generate mechanical forces that act on the ribosome and alter translation speed. Such changes in speed can affect the structure and function of the newly synthesized protein as well as cellular phenotype. The domain properties that govern force generation have yet to be identified and understood, and the influence of translation speed is unknown because all reported measurements have been carried out on arrested ribosomes. Here, using coarse-grained molecular simulations and statistical mechanical modeling of protein synthesis, we demonstrate that force generation is determined by a domain's stability and topology, as well as translation speed. The statistical mechanical models we create predict how force profiles d

SUBMITTER: Leininger SE 

PROVIDER: S-EPMC6431206 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature