Adaptive PCR Based on Hybridization Sensing of Mirror-Image l-DNA.

ABSTRACT: Polymerase chain reaction (PCR) is dependent on two key hybridization events during each cycle of amplification, primer annealing and product melting. To ensure that these hybridization events occur, current PCR approaches rely on temperature set points and reaction contents that are optimized and maintained using rigid thermal cycling programs and stringent sample preparation procedures. This report describes a fundamentally simpler and more robust PCR design that dynamically controls thermal cycling by more directly monitoring the two key hybridization events during the reaction. This is achieved by optically sensing the annealing and melting of mirror-image l-DNA analogs of the reaction's primers and targets. Because the properties of l-DNA enantiomers parallel those of natural d-DNAs,

SUBMITTER: Adams NM 

PROVIDER: S-EPMC6431534 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature