Sequencing a Juglans regia × J. microcarpa hybrid yields high-quality genome assemblies of parental species.

ABSTRACT: Members of the genus Juglans are monecious wind-pollinated trees in the family Juglandaceae with highly heterozygous genomes, which greatly complicates genome sequence assembly. The genomes of interspecific hybrids are usually comprised of haploid genomes of parental species. We exploited this attribute of interspecific hybrids to avoid heterozygosity and sequenced an interspecific hybrid Juglans microcarpa × J. regia using a novel combination of single-molecule sequencing and optical genome mapping technologies. The resulting assemblies of both genomes were remarkably complete including chromosome termini and centromere regions. Chromosome termini consisted of arrays of telomeric repeats about 8 kb long and heterochromatic subtelomeric regions about 10 kb long. The ce

SUBMITTER: Zhu T 

PROVIDER: S-EPMC6431679 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature