Functional dysbiosis within dental plaque microbiota in cleft lip and palate patients.

ABSTRACT:

Background

Dental caries is a polymicrobial disease and prevalent among cleft lip and palate (CLP) patients, although their oral hygiene is well maintained. Dysbiosis, the state of imbalance within the dental plaque microbiota, may cause caries prevalence among these patients. However, little is known about how dysbiosis occurs and affects cariogenicity. To find dysbiotic signs, here we conducted a metatranscriptomic analysis for the plaque microbiota in six CLP patients and four controls.

Methods

Total bacterial RNA was extracted from each sample and sequenced. Bacterial composition and functional profiles were estimated from 16S rRNA and mRNA reads, respectively. The mRNA reads were further used for estimating bacterial composition. Species listed in both rRNA-based and mR

SUBMITTER: Funahashi K 

PROVIDER: S-EPMC6431681 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature