Characterization of a strain of quaranfil virus isolated from soft ticks in India. Is quaranfil virus an unrecognized cause of disease in human and animals?"

ABSTRACT: The soft ticks collected during a field survey in Karnataka state, India, in 1983, yielded a novel virus isolate, which caused mortality in an infant mouse upon inoculation. Attempts at characterizing the virus using the conventional methods were unsuccessful, which prompted us to study it by Next-Generation Sequencing (NGS). This virus isolate was obtained from the viral repository of National Institute of Virology, and an initial virus stock was prepared as a mouse brain homogenate. The virus stock showed cytopathic effects in different cell-lines and was used in NGS. Based on the complete genome sequence, obtained using de novo and reference mapping approach, the virus isolate was identified as a Quaranfil virus (QRFV) belonging to the family Orthomyxoviridae, genus Quaran

SUBMITTER: Mourya DT 

PROVIDER: S-EPMC6431747 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature