The lineage-specific transcription factor CDX2 navigates dynamic chromatin to control distinct stages of intestine development.

ABSTRACT: Lineage-restricted transcription factors, such as the intestine-specifying factor CDX2, often have dual requirements across developmental time. Embryonic loss of CDX2 triggers homeotic transformation of intestinal fate, whereas adult-onset loss compromises crucial physiological functions but preserves intestinal identity. It is unclear how such diverse requirements are executed across the developmental continuum. Using primary and engineered human tissues, mouse genetics, and a multi-omics approach, we demonstrate that divergent CDX2 loss-of-function phenotypes in embryonic versus adult intestines correspond to divergent CDX2 chromatin-binding profiles in embryonic versus adult stages. CDX2 binds and activates distinct target genes in developing versus adult mouse and human intestinal cell

SUBMITTER: Kumar N 

PROVIDER: S-EPMC6432663 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature