Lateral distribution of phosphatidylinositol 4,5-bisphosphate in membranes regulates formin- and ARP2/3-mediated actin nucleation.

ABSTRACT: Spatial and temporal control of actin polymerization is fundamental for many cellular processes, including cell migration, division, vesicle trafficking, and response to agonists. Many actin-regulatory proteins interact with phosphatidylinositol 4,5-bisphosphate (PI(4,5)P₂) and are either activated or inactivated by local PI(4,5)P₂ concentrations that form transiently at the cytoplasmic face of cell membranes. The molecular mechanisms of these interactions and how the dozens of PI(4,5)P₂-sensitive actin-binding proteins are selectively recruited to membrane PI(4,5)P₂ pools remains undefined. Using a combination of biochemical, imaging, and cell biologic studies, combined with molecular dynamics and analytical theory, we test the hypothesis that t

SUBMITTER: Bucki R 

PROVIDER: S-EPMC6433049 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature