Microvesicles generated following traumatic brain injury induce platelet dysfunction via adenosine diphosphate receptor.

ABSTRACT:

Background

Traumatic brain injury (TBI) can result in an acute coagulopathy including platelet dysfunction that can contribute to ongoing intracranial hemorrhage. Previous studies have shown adenosine diphosphate (ADP)-induced platelet aggregation to be reduced after TBI. In addition, circulating microvesicles (MVs) are increased following TBI and have been shown to play a role in post-TBI coagulopathy and platelet function. We hypothesized that post-TBI MVs would affect platelet aggregation in a murine head injury model.

Methods

Moderate TBI was performed using a weight-drop method in male C57BL6 mice. Whole blood, plasma, MVs, and MV-poor plasma were isolated from blood collected 10 minutes following TBI and were mixed separately with whole blood from uninjured mice. Plate

SUBMITTER: Martin GE 

PROVIDER: S-EPMC6433504 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature