Choline kinase inhibitors EB-3D and EB-3P interferes with lipid homeostasis in HepG2 cells.

ABSTRACT: A full understanding of the molecular mechanism of action of choline kinase α (ChoKα) inhibitors at the cell level is essential for developing therapeutic and preventive approaches for cancer. The aim of the present study was to evaluate the effects of the ChoKα inhibitors EB-3D and EB-3P on lipid metabolism in HepG2 cells. We used [methyl-¹⁴C]choline, [1,2-¹⁴C]acetic acid and [2-³H]glycerol as exogenous precursors of the corresponding phospholipids and neutral lipids. [Methyl-¹⁴C]choline was also used to determine choline uptake. Protein levels were determined by Western blot. Ultrastructural alterations were investigated by transmission electron microscopy. In this work, we demonstrate that EB-3D and EB-3P interfere with phosphatidyl

SUBMITTER: Sola-Leyva A 

PROVIDER: S-EPMC6433853 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature