Porcine Gastric Mucin Triggers Toxin Production of Enteropathogenic Bacillus cereus.

ABSTRACT: Enteropathogenic Bacillus cereus causes foodborne infections due to the production of pore-forming enterotoxins in the intestine. Before that, spores have to be ingested, survive the stomach passage, and germinate. Thus, before reaching epithelial cells, B. cereus comes in contact with the intestinal mucus layer. In the present study, different aspects of this interaction were analyzed. Total RNA sequencing revealed major transcriptional changes of B. cereus strain F837/76 upon incubation with porcine gastric mucin (PGM), comprising genes encoding enterotoxins and further putative virulence factors, as well as proteins involved in adhesion to and degradation of mucin. Indeed, PGM was partially degraded by B. cereus via secreted, EDTA-sensitive proteases. The amo

SUBMITTER: Jessberger N 

PROVIDER: S-EPMC6434126 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature