Human Intestinal Enteroids as a Model System of Shigella Pathogenesis.

ABSTRACT: The enteric bacterium and intracellular human pathogen Shigella causes hundreds of millions of cases of the diarrheal disease shigellosis per year worldwide. Shigella is acquired by ingestion of contaminated food or water; upon reaching the colon, the bacteria invade colonic epithelial cells, replicate intracellularly, spread to adjacent cells, and provoke an intense inflammatory response. There is no animal model that faithfully recapitulates human disease; thus, cultured cells have been used to model Shigella pathogenesis. However, the use of transformed cells in culture does not provide the same environment to the bacteria as the normal human intestinal epithelium. Recent advances in tissue culture now enable the cultivation of human intestinal enteroids (HIEs), whi

SUBMITTER: Koestler BJ 

PROVIDER: S-EPMC6434139 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature