Selection of reference genes for the quantitative real-time PCR normalization of gene expression in Isatis indigotica fortune.

ABSTRACT: BACKGROUND:Isatis indigotica, a traditional Chinese medicine, produces a variety of active ingredients. However, little is known about the key genes and corresponding expression profiling involved in the biosynthesis pathways of these ingredients. Quantitative real-time polymerase chain reaction (qRT-PCR) is a powerful, commonly-used method for gene expression analysis, but the accuracy of the quantitative data produced depends on the appropriate selection of reference genes. RESULTS:In this study, the systematic analysis of the reference genes was performed for quantitative real-Time PCR normalization in I. indigotica. We selected nine candidate reference genes, including six traditional housekeeping genes (ACT, α-TUB, β-TUB, UBC, CYP, and EF1-α), and three newly stable internal control g

SUBMITTER: Qu R 

PROVIDER: S-EPMC6434783 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature