Quantifying normal human brain metabolism using hyperpolarized [1-¹³C]pyruvate and magnetic resonance imaging.

ABSTRACT: Hyperpolarized ¹³C Magnetic Resonance Imaging (¹³C-MRI) provides a highly sensitive tool to probe tissue metabolism in vivo and has recently been translated into clinical studies. We report the cerebral metabolism of intravenously injected hyperpolarized [1-¹³C]pyruvate in the brain of healthy human volunteers for the first time. Dynamic acquisition of ¹³C images demonstrated ¹³C-labeling of both lactate and bicarbonate, catalyzed by cytosolic lactate dehydrogenase and mitochondrial pyruvate dehydrogenase respectively. This demonstrates that both enzymes can be probed in vivo in the presence of an intact blood-brain barrier: the measured apparent exchange rate constant (k_PL) for exchange of the hyperpolarized ¹³C

SUBMITTER: Grist JT 

PROVIDER: S-EPMC6435102 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature