Molecular and antigenic characterization of Trypanosoma cruzi TolT proteins.

ABSTRACT:

Background

TolT was originally described as a Trypanosoma cruzi molecule that accumulated on the trypomastigote flagellum bearing similarity to bacterial TolA colicins receptors. Preliminary biochemical studies indicated that TolT resolved in SDS-PAGE as ~3-5 different bands with sizes between 34 and 45 kDa, and that this heterogeneity could be ascribed to differences in polypeptide glycosylation. However, the recurrent identification of TolT-deduced peptides, and variations thereof, in trypomastigote proteomic surveys suggested an intrinsic TolT complexity, and prompted us to undertake a thorough reassessment of this antigen.

Methods/principle findings

Genome mining exercises showed that TolT constitutes a larger-than-expected family of genes, with at least 12 polymorphic m

SUBMITTER: Lobo M 

PROVIDER: S-EPMC6435186 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature