Morpho-mechanics of human collagen superstructures revealed by all-optical correlative micro-spectroscopies.

ABSTRACT: In every biological tissue, morphological and topological properties strongly affect its mechanical features and behaviour, so that ultrastructure, composition and mechanical parameters are intimately connected. Overall, it is their correct interplay that guarantees the tissue functionality. The development of experimental methods able to correlate these properties would open new opportunities both in the biological and the biomedical fields. Here, we report a correlative study intended to map supramolecular morphology, biochemical composition and viscoelastic parameters of collagen by all-optical microscopies. In particular, using human corneal tissue as a benchmark, we correlate Second-Harmonic Generation maps with mechanical and biochemical imaging obtained by Brillouin and Raman micro-

SUBMITTER: Mercatelli R 

PROVIDER: S-EPMC6435656 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature