The ATP-gated P2X₁ ion channel contributes to the severity of antibody-mediated Transfusion-Related Acute Lung Injury in mice.

ABSTRACT: The biological responses that control the development of Transfusion-Related Acute Lung Injury (TRALI), a serious post-transfusion respiratory syndrome, still need to be clarified. Since extracellular nucleotides and their P2 receptors participate in inflammatory processes as well as in cellular responses to stress, we investigated the role of the ATP-gated P2X₁ cation channel in antibody-mediated TRALI. The effects of NF449, a selective P2X1 receptor (P2RX1) antagonist, were analyzed in a mouse two-hit model of TRALI. Mice were primed with lipopolysaccharide (LPS) and 24 h later challenged by administrating an anti-MHC I antibody. The selective P2RX1 antagonist NF449 was administrated before the administration of LPS and/or the anti-MHC I antibody. When given before antibody ad

SUBMITTER: El Mdawar MB 

PROVIDER: S-EPMC6435740 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature