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Label-free in vivo pathology of human epithelia with a high-speed handheld dual-axis confocal microscope.

ABSTRACT: There would be clinical value in a miniature optical-sectioning microscope to enable in vivo interrogation of tissues as a real-time and noninvasive alternative to gold-standard histopathology for early disease detection and surgical guidance. To address this need, a reflectance-based handheld line-scanned dual-axis confocal microscope was developed and fully packaged for label-free imaging of human skin and oral mucosa. This device can collect images at >15 frames/s with an optical-sectioning thickness and lateral resolution of 1.7 and 1.1 μm, respectively. Incorporation of a sterile lens cap design enables pressure-sensitive adjustment of the imaging depth by the user during clinical use. In vivo human images and videos are obtained to demonstrate the capabilities of this high-speed optical-sectioning microscopy device.

SUBMITTER: Yin C

PROVIDER: S-EPMC6435977 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature

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Label-free in vivo pathology of human epithelia with a high-speed handheld dual-axis confocal microscope.

Yin Chengbo C Wei Linpeng L Abeytunge Sanjee S Peterson Gary G Rajadhyaksha Milind M Liu Jonathan J

Journal of biomedical optics 20190327 3

There would be clinical value in a miniature optical-sectioning microscope to enable in vivo interrogation of tissues as a real-time and noninvasive alternative to gold-standard histopathology for early disease detection and surgical guidance. To address this need, a reflectance-based handheld line-scanned dual-axis confocal microscope was developed and fully packaged for label-free imaging of human skin and oral mucosa. This device can collect images at >15 frames/s with an optical-sectioning ...[more]

PMID: 32717147

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