Understanding the functions of endogenous DOF transcript factor in Chlamydomonas reinhardtii.

ABSTRACT:

Background

The regulation of genes related to lipid metabolism by genetic engineering is an important way to increase the accumulation of lipids in microalgae. DNA binding with one finger (DOF) is a plant-specific transcription factor in higher plants, where it regulates carbon and nitrogen metabolic pathways by regulating key genes involved in these pathways. Overexpression of DOF can increase lipid production in plants; however, it is not clear whether overexpression of DOF can increase lipids in microalgae.

Results

In this study, we cloned a DOF transcription factor, crDOF, from Chlamydomonas reinhardtii. The sequence of this transcription factor is 1875 bp and encodes a peptide of 624 amino acids with a conserved DOF domain. Overexpression of crDOF in C. reinha

SUBMITTER: Jia B 

PROVIDER: S-EPMC6436238 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature