Harnessing microfluidic streak plate technique to investigate the gut microbiome of Reticulitermes chinensis.

ABSTRACT: The termite gut microbiome is a model system to investigate microbial interactions and their associations with host. For decades, extensive research with molecular tools and conventional cultivation method has been carried out to define the microbial diversity in termite gut. Yet, many bacterial groups of the termite gut microbiome have not been successfully cultivated in laboratory. In this study, we adapted the recently developed microfluidic streak plate (MSP) technique for cultivation of termite gut microbial communities at both aerobic and anaerobic conditions. We found that 99 operational taxonomic units (OTUs) were cultivable by MSP approach and 18 OTUs were documented first time for termite gut microbiota. Further analysis of the bacterial diversities derived by culture-dependent M

SUBMITTER: Zhou N 

PROVIDER: S-EPMC6436436 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature