Biomedical diagnosis perspective of epigenetic detections using alpha-hemolysin nanopore.

ABSTRACT: The α-hemolysin nanopore has been studied for applications in DNA sequencing, various single-molecule detections, biomolecular interactions, and biochips. The detection of single molecules in a clinical setting could dramatically improve cancer detection and diagnosis as well as develop personalized medicine practices for patients. This brief review shortly presents the current solid state and protein nanopore platforms and their applications like biosensing and sequencing. We then elaborate on various epigenetic detections (like microRNA, G-quadruplex, DNA damages, DNA modifications) with the most widely used alpha-hemolysin pore from a biomedical diagnosis perspective. In these detections, a nanopore electrical current signature was generated by the interaction of a target with the pore.

SUBMITTER: Wang Y 

PROVIDER: S-EPMC6436813 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature