Single-Cell Transcriptome Analysis of CD34⁺ Stem Cell-Derived Myeloid Cells Infected With Human Cytomegalovirus.

ABSTRACT: Myeloid cells are important sites of lytic and latent infection by human cytomegalovirus (CMV). We previously showed that only a small subset of myeloid cells differentiated from CD34⁺ hematopoietic stem cells is permissive to CMV replication, underscoring the heterogeneous nature of these populations. The exact identity of resistant and permissive cell types, and the cellular features characterizing the latter, however, could not be dissected using averaging transcriptional analysis tools such as microarrays and, hence, remained enigmatic. Here, we profile the transcriptomes of ∼7000 individual cells at day 1 post-infection using the 10× genomics platform. We show that viral transcripts are detectable in the majority of the cells, suggesting that virion entry is unlikely to be

SUBMITTER: Galinato M 

PROVIDER: S-EPMC6437045 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature