Pseudomonas aeruginosa keratitis: passive immunotherapy with antibodies raised against divalent flagellin.

ABSTRACT:

Objectives

Pseudomonas aeruginosa infections such as keratitis are considered among the major health problems worldwide due to the complexity of pathogenesis and antibiotic resistance crisis, thus, finding new effective approaches for prevention and treatment of the infections seem to be still vital. In this report, we aimed to investigate the therapeutic effects of topical administration of the antibodies against type a and b-flagellin (FLA and FLB) in Pseudomonas keratitis model of infection in mice.

Materials and methods

Scratched corneas of mice were treated with approximately 10⁷ CFUs/eye of PAK and/or PAO1 strains of P. aeruginosa. Specific IgG to FLA, FLB or divalent flagellin were topically applied to the infected corneas for 20 min, 2

SUBMITTER: Mahin Samadi P 

PROVIDER: S-EPMC6437458 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature