Membrane metallo-endopeptidase (Neprilysin) regulates inflammatory response and insulin signaling in white preadipocytes.

ABSTRACT:

Objective

Accumulation of visceral white adipose tissue (WAT) associates with insulin resistance, adipose tissue inflammation, and metabolic syndrome, whereas accumulation of subcutaneous WAT may be protective. We aimed to identify molecular mechanisms that might provide mechanistic insights underlying the phenotypic differences in these tissues. Membrane Metallo-Endopeptidase (MME/Neprislyin) is an extracellular, membrane-bound protease enriched in subcutaneous WAT that can target degradation of a variety of peptides, including insulin, IL6, and β-amyloids. We hypothesized that MME contributes to adipose depot-specific metabolic properties.

Methods

We performed RNA sequencing on human subcutaneous and visceral preadipocytes and array gene expression profiling in murine subc

SUBMITTER: Ramirez AK 

PROVIDER: S-EPMC6437599 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature