Comprehensive and quantitative analysis of white and brown adipose tissue by shotgun lipidomics.

ABSTRACT:

Objective

Shotgun lipidomics enables an extensive analysis of lipids from tissues and fluids. Each specimen requires appropriate extraction and processing procedures to ensure good coverage and reproducible quantification of the lipidome. Adipose tissue (AT) has become a research focus with regard to its involvement in obesity-related pathologies. However, the quantification of the AT lipidome is particularly challenging due to the predominance of triacylglycerides, which elicit high ion suppression of the remaining lipid classes.

Methods

We present a new and validated method for shotgun lipidomics of AT, which tailors the lipid extraction procedure to the target specimen and features high reproducibility with a linear dynamic range of at least 4 orders of magnitude for all

SUBMITTER: Grzybek M 

PROVIDER: S-EPMC6437637 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature