Lipolysis-Permeation Setup for Simultaneous Study of Digestion and Absorption in Vitro.

ABSTRACT: Lipid-based formulations (LBFs) are a delivery strategy to enhance intestinal absorption of poorly water-soluble drugs. LBF performance is typically evaluated by in vitro lipolysis studies, but these do not accurately predict the in vivo performance. One possible reason is the absence of an absorptive membrane driving sink conditions in the serosal compartment. To explore the impact of absorption under sink conditions on the performance evaluation, we developed a lipolysis-permeation setup that allows simultaneous investigation of intestinal digestion of an LBF and drug absorption. The setup consists of two chambers, an upper one for digestion (luminal), and a lower, receiving one (serosal), separated by a Caco-2 monolayer. Digestions were performed with immobilized lipase, instead of the

SUBMITTER: Keemink J 

PROVIDER: S-EPMC6437649 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature