Feasibility of a plasma bioassay to assess oxidative protection of low-density lipoproteins by high-density lipoproteins.

ABSTRACT:

Background

Traditionally, the impact of lipoproteins on vascular disease has been evaluated in light of their quantity, that is, cholesterol content, in plasma. However, recent studies of high-density lipoproteins (HDLs) have focused on functionality with regard to atheroprotection. For example, bioassays have emerged to assess the ability of HDL, in its near native plasma environment, to promote cholesterol removal (efflux) from cells. As a result, attention has focused on developing plasma-based assays for other putative HDL protective functions including protecting low-density lipoproteins (LDLs) from oxidative damage.

Objective

To determine the feasibility of such an assay in a complex sample such as plasma, we evaluated the contribution of HDL vs other plasma factors in

SUBMITTER: Swertfeger DK 

PROVIDER: S-EPMC6437770 | biostudies-literature | 2018 Nov - Dec

REPOSITORIES: biostudies-literature